酿酒酵母菌及其灭活菌对绵羊瘤胃外植体β-防御素-1(SBD-1)表达的影响

doi:10.11843/j.issn.0366-6964.2019.03.013

Abstract

Abstract:

The aim of this study was to explore the effects of probiotic Saccharomyces cerevisiae (S.c) and inactivated S.c on the expression of sheep-defensin-1 (SBD-1) in rumen explants of sheep. After different concentrations (10⁴, 10⁵, 10⁶, 10⁷, 10⁸, 10⁹ CFU·mL^-1) of S.c and inactivated S.c were respectively co-cultured with explants for 24 h, the expression of SBD-1 mRNA and protein in rumen explants were detected by qPCR and ELISA to determine the optimal concentration for the induction of SBD-1 expression by S.c and inactivated S.c. Then, the sheep rumen explants were stimulated with the optimal concentration of S.c and inactivated S.c for 2, 4, 8, 12, 16, 20, 24 h, respectively, and the SBD-1 mRNA and protein were also detected by qPCR and ELISA, so as to screen out the optimal time for the induction of SBD-1 expression by S.c and inactivated S.c. The results showed that S.c and inactivated S.c could significantly promote the expression of SBD-1 in rumen explants (P<0.05). When the rumen explants of sheep were stimulated by the concentration of 10⁷ CFU·mL^-1 for S.c and 10⁸ CFU·mL^-1 for inactivated S.c for 16 h, respectively, the expression level of SBD-1 were the highest, and which were significantly different from the control (P<0.01). Comparing the induction effects between S.c and inactivated S.c under optimal induction conditions, the S.c were better. Therefore, S.c and inactivated S.c could promote the SBD-1 expression in rumen explants of sheep, and the expression of SBD-1 reached the maximum when the rumen explants of sheep were stimulated by the concentration of 10⁷ CFU·mL^-1 for S.c and 10⁸ CFU·mL^-1 for inactivated S.c for 16 h. The induction effect of S.c is better than that of inactivated S.c.

CLC Number:

S826

WANG Yunhe, JIN Xin, ZHANG Man, WEI Fang, WEN Jingyi, ZHAO Feifei, YANG Yinfeng. Effects of Saccharomyces Cerevisiae and Its Inactivated Bacteria on the Expression of β-defensin-1 in Ruminal Explants of Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 581-591.

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Effects of Saccharomyces Cerevisiae and Its Inactivated Bacteria on the Expression of β-defensin-1 in Ruminal Explants of Sheep

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